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RECQL4 Antibody(C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - RECQL4 Antibody(C-term) AP19458b
    All lanes : Anti-RECQL4 Antibody(C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: A431 whole cell lysate Lane 3: U-251 MG whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Mouse testis tissue lysate Lane 6: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 133 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - RECQL4 Antibody(C-term) AP19458b
    Immunohistochemical analysis of AP19458B on paraffin-embedded Human tonsil tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession O94761
Other Accession NP_004251.3
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 133067 Da
Antigen Region 1134-1162 aa
Additional Information
Gene ID 9401
Other Names ATP-dependent DNA helicase Q4, DNA helicase, RecQ-like type 4, RecQ4, RTS, RecQ protein-like 4, RECQL4, RECQ4
Target/Specificity This RECQL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1134-1162 amino acids from the C-terminal region of human RECQL4.
Dilution WB~~1:2000
IHC-P~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRECQL4 Antibody(C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RECQL4
Synonyms RECQ4 {ECO:0000303|PubMed:9878247}
Function An ATP-dependent DNA helicase which unwinds dsDNA with a 3'- overhang in a 3'-5' direction (PubMed:28653661). Does not unwind more than 18 bp of dsDNA (PubMed:28653661). May modulate chromosome segregation. The N-terminal domain (residues 1-54) binds DNA Y-shaped DNA better than ss- or dsDNA (PubMed:22730300). The core helicase domain binds ssDNA (PubMed:22730300, PubMed:28653661).
Cellular Location Cytoplasm. Nucleus
Tissue Location Ubiquitously expressed, with highest levels in thymus and testis.
Research Areas
Citations (0)
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Background

The protein encoded by this gene is a DNA helicase that belongs to the RecQ helicase family. DNA helicases unwind double-stranded DNA into single-stranded DNAs and may modulate chromosome segregation. This gene is predominantly expressed in thymus and testis. Mutations in this gene are associated with Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes. [provided by RefSeq].

References

Rossi, M.L., et al. DNA Repair (Amst.) 9(7):796-804(2010)
Singh, D.K., et al. Aging Cell 9(3):358-371(2010)
Thangavel, S., et al. Mol. Cell. Biol. 30(6):1382-1396(2010)
Xu, X., et al. EMBO J. 28(19):3005-3014(2009)
Im, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 106(37):15628-15632(2009)

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$ 365.00
$ 140.00
Cat# AP19458b
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