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ACO2 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 6
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q99798 |
| Other Accession | Q9ER34, P16276, Q99KI0, P20004 |
| Reactivity | Human, Rat |
| Predicted | Bovine, Mouse, Pig |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 85425 Da |
| Antigen Region | 438-467 aa |
| Gene ID | 50 |
|---|---|
| Other Names | Aconitate hydratase, mitochondrial, Aconitase, Citrate hydro-lyase, ACO2 |
| Target/Specificity | This ACO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-467 amino acids from the Central region of human ACO2. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ACO2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ACO2 |
|---|---|
| Function | Catalyzes the isomerization of citrate to isocitrate via cis- aconitate. |
| Cellular Location | Mitochondrion {ECO:0000250|UniProtKB:P16276}. |
Provided below are standard protocols that you may find useful for product applications.
Background
ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.
References
Juang, H.H., Mol. Genet. Metab. 81(3):244-252 (2004).
Bota, D.A., et al., Nat. Cell Biol. 4(9):674-680 (2002).
Gruer, M.J., et al., Trends Biochem. Sci. 22(1):3-6 (1997).
Klausner, R.D., et al., Mol. Biol. Cell 4(1):1-5 (1993).
Geurts van Kessel, A.H., et al., Cytogenet. Cell Genet. 28(3):169-172 (1980).
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