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Cellular Apoptosis Susceptibility Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 3
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, IF, WB, E |
|---|---|
| Primary Accession | P55060 |
| Other Accession | Q9ERK4, Q7SZC2, A5D785 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Zebrafish |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 110417 Da |
| Antigen Region | 55-84 aa |
| Gene ID | 1434 |
|---|---|
| Other Names | Exportin-2, Exp2, Cellular apoptosis susceptibility protein, Chromosome segregation 1-like protein, Importin-alpha re-exporter, CSE1L, CAS, XPO2 |
| Target/Specificity | This Cellular Apoptosis Susceptibility antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the N-terminal region of human Cellular Apoptosis Susceptibility. |
| Dilution | IF~~1:100 WB~~1:1000 IHC-P~~1:100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Cellular Apoptosis Susceptibility Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CSE1L |
|---|---|
| Synonyms | CAS {ECO:0000303|PubMed:7479798}, XPO2 |
| Function | Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. |
| Cellular Location | Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm. |
| Tissue Location | Detected in brain, placenta, ovary, testis and trachea (at protein level) (PubMed:10331944). Widely expressed (PubMed:10331944). Highly expressed in testis and in proliferating cells (PubMed:10331944, PubMed:7479798). |
Provided below are standard protocols that you may find useful for product applications.
Background
Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. CSE1L binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, CSE1L may play a role both in apoptosis and in cell proliferation.
References
Goldberg, G.S., et al., J. Biol. Chem. 278(47):46533-46540 (2003).
Behrens, P., et al., Apoptosis 8(1):39-44 (2003).
Jiang, M.C., et al., Biochem. Biophys. Res. Commun. 294(4):900-905 (2002).
Wellmann, A., et al., Int. J. Mol. Med. 7(5):489-494 (2001).
Brinkmann, U., et al., Genomics 58(1):41-49 (1999).
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