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RCC2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RCC2 Antibody (Center) AP19348c
    RCC2 Antibody (Center)(Cat. #AP19348c) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RCC2 antibody detected the RCC2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9P258
Other Accession NP_061185.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 56085 Da
Antigen Region 169-198 aa
Additional Information
Gene ID 55920
Other Names Protein RCC2, RCC1-like protein TD-60, Telophase disk protein of 60 kDa, RCC2, KIAA1470, TD60
Target/Specificity This RCC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-198 amino acids from the Central region of human RCC2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRCC2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RCC2
Synonyms KIAA1470, TD60
Function Multifunctional protein that may affect its functions by regulating the activity of small GTPases, such as RAC1 and RALA (PubMed:12919680, PubMed:25074804, PubMed:26158537, PubMed:28869598). Required for normal progress through the cell cycle, both during interphase and during mitosis (PubMed:12919680, PubMed:23388455, PubMed:26158537). Required for the presence of normal levels of MAD2L1, AURKB and BIRC5 on inner centromeres during mitosis, and for normal attachment of kinetochores to mitotic spindles (PubMed:12919680, PubMed:26158537). Required for normal organization of the microtubule cytoskeleton in interphase cells (PubMed:23388455). Functions as guanine nucleotide exchange factor (GEF) for RALA (PubMed:26158537). Interferes with the activation of RAC1 by guanine nucleotide exchange factors (PubMed:25074804). Prevents accumulation of active, GTP-bound RAC1, and suppresses RAC1-mediated reorganization of the actin cytoskeleton and formation of membrane protrusions (PubMed:25074804, PubMed:28869598). Required for normal cellular responses to contacts with the extracellular matrix of adjacent cells, and for directional cell migration in response to a fibronectin gradient (in vitro) (PubMed:25074804, PubMed:28869598).
Cellular Location Nucleus, nucleolus. Nucleus. Cytoplasm, cytoskeleton. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome. Midbody. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex (PubMed:12919680, PubMed:1939370, PubMed:7559776, PubMed:9914378). Colocalizes with cytoplasmic microtubules in interphase cells (PubMed:23388455). Colocalizes with RAC1 at the cell membrane (PubMed:25074804).
Research Areas
Citations (0)
citation

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Background

Required for completion of mitosis and cytokinesis. RCC2 may function as a guanine nucleotide exchange factor for the small GTPase RAC1.

References

Humphries, J.D., et al. Sci Signal 2 (87), RA51 (2009) :
Stacey, S.N., et al. Nat. Genet. 40(11):1313-1318(2008)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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$ 182.50
$ 70.00
Cat# AP19348c
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