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Isocitrate dehydrogenase (IDH3) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Isocitrate dehydrogenase (IDH3) Antibody (C-term) AP1927A
    Western blot analysis of Isocitrate dehydrogenase (Cat. #AP1927a) in MCF-7, Jurkat cell line lysates (35ug/lane). Isocitrate dehydrogenase (arrow) was detected using the purified Pab.
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  • SPECIFICATION
  • CITATIONS: 1
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P50213
Other Accession Q99NA5, Q9D6R2, Q28480, P41563, P56471
Reactivity Human
Predicted Bovine, Monkey, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 39592 Da
Antigen Region 317-346 aa
Additional Information
Gene ID 3419
Other Names Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, Isocitric dehydrogenase subunit alpha, NAD(+)-specific ICDH subunit alpha, IDH3A
Target/Specificity This Isocitrate dehydrogenase (IDH3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human Isocitrate dehydrogenase (IDH3).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIsocitrate dehydrogenase (IDH3) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IDH3A (HGNC:5384)
Function Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.
Cellular Location Mitochondrion.
Research Areas
Citations ( 0 )

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein described here is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.

References

Soundar, S., et al., J. Biol. Chem. 278(52):52146-52153 (2003).
Weiss, C., et al., Biochemistry 39(7):1807-1816 (2000).
Kim, Y.O., et al., J. Biol. Chem. 274(52):36866-36875 (1999).
Huh, T.L., et al., Genomics 32(2):295-296 (1996).
Kim, Y.O., et al., Biochem. J. 308 (PT 1), 63-68 (1995) (): ().

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$ 365.00
$ 140.00
Cat# AP1927A
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