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NCAPG Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NCAPG Antibody (N-term) AP19113a
    NCAPG Antibody (N-term) (Cat. #AP19113a) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NCAPG antibody detected the NCAPG protein (arrow).
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  • CITATIONS: 1
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9BPX3
Other Accession NP_071741.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 114334 Da
Antigen Region 117-145 aa
Additional Information
Gene ID 64151
Other Names Condensin complex subunit 3, Chromosome-associated protein G, Condensin subunit CAP-G, hCAP-G, Melanoma antigen NY-MEL-3, Non-SMC condensin I complex subunit G, XCAP-G homolog, NCAPG, CAPG, NYMEL3
Target/Specificity This NCAPG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-145 amino acids from the N-terminal region of human NCAPG.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNCAPG Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCAPG
Synonyms CAPG, NYMEL3
Function Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Cellular Location Nucleus. Cytoplasm. Chromosome. Note=In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDK1, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase
Tissue Location Highly expressed in testis.
Research Areas
Citations ( 0 )

Background

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

References

Okada, Y., et al. Hum. Mol. Genet. 19(11):2303-2312(2010)
Zhao, J., et al. BMC Med. Genet. 11, 96 (2010) :
Murphy, L.A., et al. Biochem. Biophys. Res. Commun. 377(3):1007-1011(2008)
Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)

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$ 182.50
$ 70.00
Cat# AP19113a
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