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RALA Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RALA Antibody (C-term) AP18995b
    RALA Antibody (C-term) (Cat. #AP18995b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RALA antibody detected the RALA protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P11233
Other Accession P63322, P63321, NP_005393.2
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 23567 Da
Antigen Region 158-186 aa
Additional Information
Gene ID 5898
Other Names Ras-related protein Ral-A, RALA, RAL
Target/Specificity This RALA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the C-terminal region of human RALA.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRALA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RALA
Synonyms RAL
Function Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors (PubMed:18756269, PubMed:19306925, PubMed:20005108, PubMed:21822277, PubMed:30500825). Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. The RALA- exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling (PubMed:20005108). Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage- independent proliferation of transformed cells (PubMed:19306925). During mitosis, supports the stabilization and elongation of the intracellular bridge between dividing cells. Cooperates with EXOC2 to recruit other components of the exocyst to the early midbody (PubMed:18756269). During mitosis, also controls mitochondrial fission by recruiting to the mitochondrion RALBP1, which mediates the phosphorylation and activation of DNM1L by the mitotic kinase cyclin B- CDK1 (PubMed:21822277).
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Cleavage furrow. Midbody, Midbody ring. Mitochondrion. Note=Predominantly at the cell surface in the absence of LPA. In the presence of LPA, colocalizes with LPAR1 and LPAR2 in endocytic vesicles (PubMed:19306925). May colocalize with CNTRL/centriolin at the midbody ring (PubMed:16213214). However, localization at the midbody at late cytokinesis was not confirmed (PubMed:18756269). Relocalizes to the mitochondrion during mitosis where it regulates mitochondrial fission (PubMed:21822277)
Research Areas
Citations (0)
citation

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Background

The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins.

References

Nichols, C.D., et al. Curr. Biol. 20(14):1316-1320(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Godin, C.M., et al. Mol. Pharmacol. 77(3):388-395(2010)
Lim, K.H., et al. Mol. Cell. Biol. 30(2):508-523(2010)
Wang, K., et al. Int J Immunopathol Pharmacol 22(3):735-743(2009)

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$ 182.50
$ 70.00
Cat# AP18995b
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