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FLOT1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FLOT1 Antibody (N-term) AP18905a
    FLOT1 Antibody (N-term)(Cat. #AP18905a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FLOT1 antibody detected the FLOT1 protein (arrow).
    detail
  • WB - FLOT1 Antibody (N-term) AP18905a
    All lanes : Anti-FLOT1 Antibody (N-term) at 1:1000 dilution Lane 1: SK-OV-3 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O75955
Other Accession Q9Z1E1, Q767L6, O08917, Q08DN8, NP_005794.1
Reactivity Human
Predicted Bovine, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 47355 Da
Antigen Region 104-133 aa
Additional Information
Gene ID 10211
Other Names Flotillin-1, FLOT1
Target/Specificity This FLOT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the N-terminal region of human FLOT1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFLOT1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FLOT1
Function May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Cellular Location Cell membrane; Peripheral membrane protein. Endosome Membrane, caveola {ECO:0000250|UniProtKB:O08917}; Peripheral membrane protein {ECO:0000250|UniProtKB:O08917}. Melanosome. Membrane raft. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065) Membrane-associated protein of caveola (By similarity) {ECO:0000250|UniProtKB:O08917, ECO:0000269|PubMed:17081065}
Research Areas
Citations (0)
citation

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Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq].

References

Lee, B.Y., et al. Mol. Cell Proteomics 9(1):32-53(2010)
Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) :
Saalik, P., et al. Bioconjug. Chem. 20(5):877-887(2009)
Rajendran, L., et al. PLoS ONE 4 (12), E8290 (2009) :
Rossy, J., et al. PLoS ONE 4 (4), E5403 (2009) :

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$ 365.00
$ 140.00
Cat# AP18905a
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