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ZHX2 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - ZHX2 Antibody (N-term) AP18752A
    Fluorescent confocal image of A549 cell stained with ZHX2 Antibody (N-term)(Cat#AP18752a).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZHX2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZHX2 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.
    detail
  • WB - ZHX2 Antibody (N-term) AP18752A
    ZHX2 Antibody (N-term)(Cat. #AP18752a) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ZHX2 antibody detected the ZHX2 protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, WB, E
Primary Accession Q9Y6X8
Other Accession Q8C0C0, NP_055758.1, Q80VX4
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 92307 Da
Antigen Region 105-131 aa
Additional Information
Gene ID 22882
Other Names Zinc fingers and homeoboxes protein 2, Alpha-fetoprotein regulator 1, AFP regulator 1, Regulator of AFP, Zinc finger and homeodomain protein 2, ZHX2, AFR1, KIAA0854, RAF
Target/Specificity This ZHX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-131 amino acids from the N-terminal region of human ZHX2.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZHX2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZHX2
Synonyms AFR1, KIAA0854, RAF
Function Acts as a transcriptional repressor (PubMed:12741956). Represses the promoter activity of the CDC25C gene stimulated by NFYA (PubMed:12741956). May play a role in retinal development where it regulates the composition of bipolar cell populations, by promoting differentiation of bipolar OFF-type cells (By similarity). In the brain, may promote maintenance and suppress differentiation of neural progenitor cells in the developing cortex (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000269|PubMed:12741956, ECO:0000269|PubMed:17056598} Note=Colocalizes with EFNB1 intracellular domain in the nucleus {ECO:0000250|UniProtKB:Q8C0C0}
Tissue Location Ubiquitously expressed. Expressed in podocytes.
Research Areas
Citations (0)
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Background

The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Xu, J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(5):2136-2140(2010)
Trynka, G., et al. Gut 58(8):1078-1083(2009)
Shen, H., et al. J. Cell. Mol. Med. 12 (6B), 2772-2780 (2008) :
Hu, S., et al. Neoplasma 54(3):207-211(2007)

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$ 182.50
$ 70.00
Cat# AP18752A
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