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GDE1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GDE1 Antibody (Center) AP18638c
    GDE1 Antibody (Center) (Cat. #AP18638c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GDE1 antibody detected the GDE1 protein (arrow).
    detail
  • WB - GDE1 Antibody (Center) AP18638c
    Anti-GDE1 Antibody (Center) at 1:1000 dilution + Rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - GDE1 Antibody (Center) AP18638c
    Overlay histogram showing Hela cells stained with AP18638c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP18638c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q9NZC3
Other Accession NP_057725.1
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 37718 Da
Antigen Region 192-219 aa
Additional Information
Gene ID 51573
Other Names Glycerophosphodiester phosphodiesterase 1, Membrane-interacting protein of RGS16, RGS16-interacting membrane protein, GDE1, MIR16
Target/Specificity This GDE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-219 amino acids of human GDE1.
Dilution WB~~1:1000
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGDE1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GDE1 (HGNC:29644)
Function Hydrolyzes the phosphodiester bond of glycerophosphodiesters such as glycerophosphoinositol (GroPIns) and glycerophosphoethanolamine (GroPEth), to yield a glycerol phosphate and an alcohol (By similarity). Hydrolyzes glycerophospho-N-acylethanolamines to N- acylethanolamines in the brain and participates in bioactive N- acylethanolamine biosynthesis such as anandamide (an endocannabinoid), N-palmitoylethanolamine (an anti-inflammatory), and N- oleoylethanolamine (an anorexic). In addition, has a lysophospholipase D activity by hydrolyzing N-acyl-lysoplasmenylethanolamine (N-acyl- lysoPlsEt) to N-acylethanolamine. However lysophospholipase D activity is lower than glycerophosphodiester phosphodiesterase activity (By similarity). Has little or no activity towards glycerophosphocholine (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:Q9JL55}; Multi-pass membrane protein. Cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:Q9JL55}; Multi-pass membrane protein. Note=Perinuclear vesicles and cell membrane {ECO:0000250|UniProtKB:Q9JL55}
Tissue Location Widely expressed..
Research Areas
Citations (0)
citation

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Background

GDE1 has glycerophosphoinositol phosphodiesterase activity. Has little or no activity towards glycerophosphocholine. GDE1 activity can be modulated by G-protein signaling pathways (By similarity).

References

Zhang, X., et al. Cancer Res. 70(18):7176-7186(2010)
Corthals, S.L., et al. Leuk. Res. 34(5):677-681(2010)
Simon, G.M., et al. J. Biol. Chem. 283(14):9341-9349(2008)
Ma, J., et al. Atherosclerosis 191(1):63-72(2007)
Bachmann, A.S., et al. Gene 371(1):144-153(2006)

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$ 182.50
$ 70.00
Cat# AP18638c
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