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UVRAG Antibody (L133)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - UVRAG Antibody (L133) AP1850d
    Immunofluorescence staining of Autophagy UVRAG antibody (Cat# AP1850d) on Methanol-fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.
    detail
  • WB - UVRAG Antibody (L133) AP1850d
    Western blot analysis of anti-hUVRAG (Center) Pab (RB10846) in mouse liver tissue lysate. hUVRAG (Center)(arrow) was detected using the purified Pab.
    detail
  • IHC-P - UVRAG Antibody (L133) AP1850d
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with hUVRAG (N-term L133), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, IF, E
Primary Accession Q9P2Y5
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 78151 Da
Antigen Region 120-152 aa
Additional Information
Gene ID 7405
Other Names UV radiation resistance-associated gene protein, p63, UVRAG
Target/Specificity This UVRAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-152 amino acids from human UVRAG.
Dilution IF~~Tested
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUVRAG Antibody (L133) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UVRAG
Function Versatile protein that is involved in regulation of different cellular pathways implicated in membrane trafficking. Involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; the function is dependent on its binding to phosphatidylinositol 3- phosphate (PtdIns(3)P) (PubMed:16799551, PubMed:18552835, PubMed:20643123, PubMed:24056303, PubMed:28306502). During autophagy acts as a regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. Activates lipid kinase activity of PIK3C3 (PubMed:16799551, PubMed:20643123, PubMed:24056303, PubMed:28306502). Involved in the regulation of degradative endocytic trafficking and cytokinesis, and in regulation of ATG9A transport from the Golgi to the autophagosome; the functions seems to implicate its association with PI3KC3-C2 (PubMed:16799551, PubMed:20643123, PubMed:24056303). Involved in maturation of autophagosomes and degradative endocytic trafficking independently of BECN1 but depending on its association with a class C Vps complex (possibly the HOPS complex); the association is also proposed to promote autophagosome recruitment and activation of Rab7 and endosome-endosome fusion events (PubMed:18552835, PubMed:28306502). Enhances class C Vps complex (possibly HOPS complex) association with a SNARE complex and promotes fusogenic SNARE complex formation during late endocytic membrane fusion (PubMed:24550300). In case of negative- strand RNA virus infection is required for efficient virus entry, promotes endocytic transport of virions and is implicated in a VAMP8- specific fusogenic SNARE complex assembly (PubMed:24550300).
Cellular Location Late endosome. Lysosome. Cytoplasmic vesicle, autophagosome. Early endosome. Endoplasmic reticulum. Midbody. Chromosome, centromere. Note=Colocalizes with RAB9-positive compartments involved in retrograde transport from late endosomes to trans-Golgi network. Colocalization with early endosomes is only partial (PubMed:24056303). Recruited to autophagosome following interaction with RUBCNL/PACER (PubMed:28306502)
Tissue Location Highly expressed in brain, lung, kidney and liver.
Research Areas
Citations (0)
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Background

UVRAG complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving this gene are associated with left-right axis malformation and mutations in this gene have been associated with colon cancer.

References

Liang,C., et al. Nat. Cell Biol. 8 (7), 688-699 (2006)
Ionov,Y., et al. Oncogene 23 (3), 639-645 (2004)
Goi,T., et al. Surg. Today 33 (9), 702-706 (2003)
Iida,A., et al. Hum. Genet. 106 (3), 277-287 (2000)
Perelman,B., et al. Genomics 41 (3), 397-405 (1997)
Bekri,S., et al. Cytogenet. Cell Genet. 79 (1-2), 125-131 (1997)
Teitz,T., et al. Gene 87 (2), 295-298 (1990)

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$ 182.50
$ 70.00
Cat# AP1850d
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