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RAB20 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RAB20 Antibody (C-term) AP17593b
    RAB20 Antibody (C-term) (Cat. #AP17593b) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the RAB20 antibody detected the RAB20 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9NX57
Other Accession NP_060287.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 26277 Da
Antigen Region 202-229 aa
Additional Information
Gene ID 55647
Other Names Ras-related protein Rab-20, RAB20
Target/Specificity This RAB20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-229 amino acids from the C-terminal region of human RAB20.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB20 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB20
Function Plays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes.
Cellular Location Golgi apparatus. Cytoplasmic vesicle, phagosome Cytoplasmic vesicle, phagosome membrane; Lipid-anchor; Cytoplasmic side. Note=Highly enriched on apical endocytic structures in polarized epithelial cells of kidney proximal tubules (By similarity). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211) {ECO:0000250|UniProtKB:P35295, ECO:0000269|PubMed:21255211}
Tissue Location Low or absent expression in normal pancreas and stronger expression in 15 of 18 exocrine pancreatic adenocarcinomas (at protein level).
Research Areas
Citations (0)
citation

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Background

RAB20 plays a role in apical endocytosis/recycling.

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Das Sarma, J., et al. Cell Commun. Adhes. 15(1):65-74(2008)
Amillet, J.M., et al. Hum. Pathol. 37(3):256-263(2006)
Pereira-Leal, J.B., et al. J. Mol. Biol. 313(4):889-901(2001)
Lutcke, A., et al. J. Cell. Sci. 107 (PT 12), 3437-3448 (1994) :

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$ 182.50
$ 70.00
Cat# AP17593b
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