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TCF25 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, E |
|---|---|
| Primary Accession | Q9BQ70 |
| Other Accession | Q8R3L2, NP_055787.1 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 76667 Da |
| Antigen Region | 186-215 aa |
| Gene ID | 22980 |
|---|---|
| Other Names | Transcription factor 25, TCF-25, Nuclear localized protein 1, TCF25, KIAA1049, NULP1 |
| Target/Specificity | This TCF25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the N-terminal region of human TCF25. |
| Dilution | IF~~1:10~50 WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | TCF25 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TCF25 {ECO:0000303|PubMed:30244831, ECO:0000312|HGNC:HGNC:29181} |
|---|---|
| Function | Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation (PubMed:30244831). In the RQC complex, required to promote formation of 'Lys-48'-linked polyubiquitin chains during ubiquitination of incompletely synthesized proteins by LTN1 (PubMed:30244831). Also acts as a transcriptional repressor: represses transcription of SRF in vitro and so may play a role in heart development (PubMed:16574069). May play a role in cell death control (By similarity). |
| Cellular Location | Nucleus. Cytoplasm, cytosol. Note=Mainly nuclear |
| Tissue Location | In the embryo, widely expressed with highest levels in brain. In the adult, highest expression is found in the heart |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
TCF25 is a member of the basic helix-loop-helix (bHLH) family of transcription factors that are important in embryonic development (Steen and Lindholm, 2008 [PubMed 18068114]).[supplied by OMIM].
References
Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Steen, H., et al. Biochem. Biophys. Res. Commun. 366(2):432-437(2008)
Cai, Z., et al. Biochem. Biophys. Res. Commun. 343(3):973-981(2006)
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