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UBA6 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | A0AVT1 |
| Other Accession | Q8C7R4, NP_060697.4 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 117970 Da |
| Antigen Region | 861-889 aa |
| Gene ID | 55236 |
|---|---|
| Other Names | Ubiquitin-like modifier-activating enzyme 6, Ubiquitin-activating enzyme 6, Monocyte protein 4, MOP-4, Ubiquitin-activating enzyme E1-like protein 2, E1-L2, UBA6, MOP4, UBE1L2 |
| Target/Specificity | This UBA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 861-889 amino acids from the C-terminal region of human UBA6. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | UBA6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | UBA6 |
|---|---|
| Synonyms | MOP4, UBE1L2 |
| Function | Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility. |
| Tissue Location | Widely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis. |
Provided below are standard protocols that you may find useful for product applications.
Background
Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many signaling networks and requires a ubiquitin-activating enzyme (E1), a ubiquitin conjugating enzyme (E2), and a ubiquitin protein ligase (E3). UBE1L2 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins (Jin et al., 2007 [PubMed 17597759]).
References
Groettrup, M., et al. Trends Biochem. Sci. 33(5):230-237(2008)
Chiu, Y.H., et al. Mol. Cell 27(6):1014-1023(2007)
Pelzer, C., et al. J. Biol. Chem. 282(32):23010-23014(2007)
Jin, J., et al. Nature 447(7148):1135-1138(2007)
Hillier, L.W., et al. Nature 434(7034):724-731(2005)
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