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NCAPD3 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P42695 |
| Other Accession | NP_056076.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 168891 Da |
| Antigen Region | 1050-1078 aa |
| Gene ID | 23310 |
|---|---|
| Other Names | Condensin-2 complex subunit D3, Non-SMC condensin II complex subunit D3, hCAP-D3, NCAPD3, CAPD3, KIAA0056 |
| Target/Specificity | This NCAPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1050-1078 amino acids from the C-terminal region of human NCAPD3. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NCAPD3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NCAPD3 {ECO:0000303|PubMed:27737959, ECO:0000312|HGNC:HGNC:28952} |
|---|---|
| Function | Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis (PubMed:14532007). May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Specifically required for decatenation of centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size (PubMed:27737959). |
| Cellular Location | Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 (MIM 605576) and SMC4 (MIM 605575), but they contain different sets of non-SMC subunits. NCAPD3 is 1 of 3 non-SMC subunits that define condensin II (Ono et al., 2003 [PubMed 14532007]).
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Takemoto, A., et al. Nat. Struct. Mol. Biol. 16(12):1302-1308(2009)
Lapointe, J., et al. Am. J. Surg. Pathol. 32(2):205-209(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Seshadri, S., et al. BMC Med. Genet. 8 SUPPL 1, S15 (2007) :
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