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ELK3 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - ELK3 Antibody (N-term) AP16475a
    ELK3 Antibody (N-term) (Cat. #AP16475a) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the ELK3 antibody detected the ELK3 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P41970
Other Accession NP_005221.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 44240 Da
Antigen Region 98-126 aa
Additional Information
Gene ID 2004
Other Names ETS domain-containing protein Elk-3, ETS-related protein ERP, ETS-related protein NET, Serum response factor accessory protein 2, SAP-2, SRF accessory protein 2, ELK3, NET, SAP2
Target/Specificity This ELK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the N-terminal region of human ELK3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsELK3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ELK3
Synonyms NET, SAP2
Function May be a negative regulator of transcription, but can activate transcription when coexpressed with Ras, Src or Mos. Forms a ternary complex with the serum response factor and the ETS and SRF motifs of the Fos serum response element.
Cellular Location Nucleus.
Research Areas
Citations (0)
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Background

The protein encoded by this gene is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present.

References

Serchov, T., et al. J. Biol. Chem. 285(28):21223-21232(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Li, B., et al. Tohoku J. Exp. Med. 216(2):139-147(2008)
Wasylyk, C., et al. Cancer Res. 68(5):1275-1283(2008)
Wu, C., et al. Proteomics 7(11):1775-1785(2007)

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$ 365.00
$ 140.00
Cat# AP16475a
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