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RPL12 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P30050 |
| Other Accession | P23358, P35979, P61284, NP_000967.1, G1SMR7 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Rabbit, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 17819 Da |
| Antigen Region | 71-100 aa |
| Gene ID | 6136 |
|---|---|
| Other Names | 60S ribosomal protein L12, RPL12 |
| Target/Specificity | This RPL12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-100 amino acids from the Central region of human RPL12. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RPL12 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RPL12 |
|---|---|
| Function | Component of the large ribosomal subunit (PubMed:25901680). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:25901680). Binds directly to 26S ribosomal RNA (PubMed:25901680). |
| Cellular Location | Cytoplasm |
Provided below are standard protocols that you may find useful for product applications.
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L11P family of ribosomal proteins. It is located in the cytoplasm. The protein binds directly to the 26S rRNA. This gene is co-transcribed with the U65 snoRNA, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
References
Letra, A., et al. Am. J. Med. Genet. A 152A (7), 1701-1710 (2010) :
Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009) :
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
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