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Mouse Cdk8 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q8R3L8 |
| Other Accession | P49336, Q8JH47, NP_705827.2 |
| Reactivity | Human |
| Predicted | Zebrafish |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 53210 Da |
| Antigen Region | 245-272 aa |
| Gene ID | 264064 |
|---|---|
| Other Names | Cyclin-dependent kinase 8, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Cdk8 |
| Target/Specificity | This Mouse Cdk8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 245-272 amino acids from the Central region of mouse Cdk8. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Cdk8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Cdk8 |
|---|---|
| Function | Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C- terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation (By similarity). |
| Cellular Location | Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation (By similarity).
References
Alarcon, C., et al. Cell 139(4):757-769(2009)
Westerling, T., et al. Mol. Cell. Biol. 27(17):6177-6182(2007)
Jang, M.K., et al. Mol. Cell 19(4):523-534(2005)
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003)
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