MAFA Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q8NHW3 |
Other Accession | Q8CF90, NP_963883.2, D3ZNT6 |
Reactivity | Human |
Predicted | Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 36982 Da |
Antigen Region | 286-315 aa |
Gene ID | 389692 |
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Other Names | Transcription factor MafA, Pancreatic beta-cell-specific transcriptional activator, Transcription factor RIPE3b1, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, MAFA |
Target/Specificity | This MAFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human MAFA. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | MAFA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MAFA |
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Function | Transcription factor that activates insulin gene expression (PubMed:12011435, PubMed:15993959). Acts synergistically with NEUROD1/BETA2 and PDX1 (PubMed:15993959). Binds the insulin enhancer C1/RIPE3b element (PubMed:12011435). Binds to consensus TRE-type MARE 5'-TGCTGACTCAGCA-3' DNA sequence (PubMed:23148532, PubMed:29339498). |
Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00978, ECO:0000269|PubMed:12011435, ECO:0000269|PubMed:12917329} |
Tissue Location | Expressed in the islets of Langerhans (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
Background
MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).
References
Lecoin, L., et al. Dev Neurobiol 70(7):485-497(2010)
Guo, S., et al. J. Biol. Chem. 285(17):12655-12661(2010)
Kondo, T., et al. Mol. Endocrinol. 23(8):1281-1290(2009)
Kaneto, H., et al. Adv. Drug Deliv. Rev. 61 (7-8), 489-496 (2009) :
Garin, I., et al. Diabet. Med. 26(7):746-748(2009)
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