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GJB2 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P29033 |
| Other Accession | A2VE67, P46691 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 26215 Da |
| Antigen Region | 204-226 aa |
| Gene ID | 2706 |
|---|---|
| Other Names | Gap junction beta-2 protein, Connexin-26, Cx26, GJB2 |
| Target/Specificity | This GJB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-226 amino acids from the C-terminal region of human GJB2. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GJB2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GJB2 |
|---|---|
| Function | Structural component of gap junctions (PubMed:16849369, PubMed:17551008, PubMed:19340074, PubMed:19384972, PubMed:21094651, PubMed:26753910). Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane (PubMed:17551008, PubMed:19340074, PubMed:21094651, PubMed:26753910). Small molecules and ions diffuse from one cell to a neighboring cell via the central pore (PubMed:16849369, PubMed:19384972, PubMed:21094651). |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. Note=Colocalizes with GJB4 at gap junction plaques in the cochlea. {ECO:0000250|UniProtKB:Q00977} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. Immunohistochemical staining of human cochlear cells demonstrated high levels of GJB2 expression. Mutations in GJB2 are associated with genetically derived hearing impairments, including autosomal recessive nonsyndromic deafness.
References
Ohtsuka, A., et al., Hum. Genet. 112(4):329-333 (2003).
Yotsumoto, S., et al., Br. J. Dermatol. 148(4):649-653 (2003).
Uyguner, O., et al., Clin. Genet. 62(4):306-309 (2002).
Richard, G., et al., Am. J. Hum. Genet. 70(5):1341-1348 (2002).
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
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