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Connexin 43 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 8
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P17302 |
| Other Accession | P16863, P18246 |
| Reactivity | Human, Mouse, Rat |
| Predicted | Bovine, Xenopus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 43008 Da |
| Antigen Region | 107-138 aa |
| Gene ID | 2697 |
|---|---|
| Other Names | Gap junction alpha-1 protein, Connexin-43, Cx43, Gap junction 43 kDa heart protein, GJA1, GJAL |
| Target/Specificity | This Connexin 43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-138 amino acids from the N-terminal region of human Connexin 43. |
| Dilution | WB~~1:2000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Connexin 43 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GJA1 |
|---|---|
| Synonyms | GJAL |
| Function | Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity). |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. Endoplasmic reticulum {ECO:0000250|UniProtKB:P23242}. Note=Localizes at the intercalated disk (ICD) in cardiomyocytes and the proper localization at ICD is dependent on TMEM65. {ECO:0000250|UniProtKB:P23242} |
| Tissue Location | Expressed in the heart and fetal cochlea. |
Provided below are standard protocols that you may find useful for product applications.
Background
Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.
References
Spinella, F., et al., J. Biol. Chem. 278(42):41294-41301 (2003). Contreras, J.E., et al., Proc. Natl. Acad. Sci. U.S.A. 100(20):11388-11393 (2003). Qin, H., et al., J. Biol. Chem. 278(32):30005-30014 (2003). Cameron, S.J., et al., J. Biol. Chem. 278(20):18682-18688 (2003). Ma, X.D., et al., World J. Gastroenterol. 9(5):946-950 (2003).
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