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TCF12 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - TCF12 Antibody (N-term) AP14835a
    TCF12 Antibody (N-term) (Cat.# AP14835a) western blot analysis in Jurkat cell line and mouse kidney lysates (35ug/lane).This demonstrates the TCF12 antibody detected the TCF12 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q99081
Other Accession P51514, Q61286, NP_996923.1, NP_996919.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 72965 Da
Antigen Region 55-84 aa
Additional Information
Gene ID 6938
Other Names Transcription factor 12, TCF-12, Class B basic helix-loop-helix protein 20, bHLHb20, DNA-binding protein HTF4, E-box-binding protein, Transcription factor HTF-4, TCF12, BHLHB20, HEB, HTF4
Target/Specificity This TCF12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the N-terminal region of human TCF12.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTCF12 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TCF12
Synonyms BHLHB20, HEB, HTF4
Function Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3') (By similarity). May be involved in the functional network that regulates the development of the GnRH axis (PubMed:32620954).
Cellular Location Nucleus.
Tissue Location Expressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line
Research Areas
Citations (0)
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Background

The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq].

References

Schotte, R., et al. J. Immunol. 184(12):6670-6679(2010)
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Landa, I., et al. PLoS Genet. 5 (9), E1000637 (2009) :
Gardini, A., et al. PLoS Genet. 4 (11), E1000275 (2008) :
Kumar, R., et al. J. Biol. Chem. 283(27):19026-19038(2008)

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$ 365.00
$ 140.00
Cat# AP14835a
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