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Alkaline Phosphatase (ALPI) Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
_(Center)_-_HeLa.jpg)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | P09923 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 56812 Da |
| Antigen Region | 274-304 aa |
| Gene ID | 248 |
|---|---|
| Other Names | Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, ALPI |
| Target/Specificity | This Alkaline Phosphatase (ALPI) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-304 amino acids from the Central region of human Alkaline Phosphatase (ALPI). |
| Dilution | IF~~1:100 WB~~1:1000 IHC-P~~1:100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Alkaline Phosphatase (ALPI) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ALPI |
|---|---|
| Function | Alkaline phosphatase that can hydrolyze various phosphate compounds. |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P15693}; Lipid-anchor, GPI-anchor {ECO:0000250|UniProtKB:P15693} |
Provided below are standard protocols that you may find useful for product applications.
Background
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.
References
References for protein:
1.Torres,M.I., Pathol. Res. Pract. 203 (6), 485-487 (2007)
2.Olsen,L., Am. J. Physiol. Gastrointest. Liver Physiol. 289 (2), G220-G226(2005)
3.Alkhoury,F., Am. J. Physiol. Gastrointest. Liver Physiol. 289 (2), G285-G290(2005)
References for HeLa cell line:
1. Scherer WF, Syverton JT, Gey GO (May 1953). "Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix". J. Exp. Med. 97 (5): 695–710. [PubMed:13052828].
2. Macville M, Schröck E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T (January 1999). "Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping". Cancer Res. 59 (1): 141–50. [PubMed: 9892199].
3. Rahbari R, Sheahan T, Modes V, Collier P, Macfarlane C, Badge RM (April 2009). "A novel L1 retrotransposon marker for HeLa cell line identification". BioTechniques 46 (4): 277–84. [PubMed: 19450234].
4. Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (July 2010). "Check your cultures! A list of cross-contaminated or misidentified cell lines". Int. J. Cancer 127 (1): 1–8. [PubMed:20143388].
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