MCM6 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
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Primary Accession | Q14566 |
Other Accession | Q2KIZ8, NP_005906.2 |
Reactivity | Human |
Predicted | Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 92889 Da |
Antigen Region | 80-109 aa |
Gene ID | 4175 |
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Other Names | DNA replication licensing factor MCM6, p105MCM, MCM6 |
Target/Specificity | This MCM6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-109 amino acids from the N-terminal region of human MCM6. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | MCM6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MCM6 (HGNC:6949) |
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Function | Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:16899510, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232, PubMed:9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425). |
Cellular Location | Nucleus. Chromosome. Note=Binds to chromatin during G1 and detaches from it during S phase. |
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Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.
References
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Timpson, N.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1341-1348(2010)
Wei, Z., et al. J. Biol. Chem. 285(17):12469-12473(2010)
Upton, J., et al. N. Z. Med. J. 123 (1308), 123 (2010) :
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
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