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Latexin Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Latexin Antibody (Center) AP1467c
    Western blot analysis of Latexin (arrow) using rabbit polyclonal Latexin Antibody (Center) (RB12661). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LXN gene (Lane 2) (Origene Technologies).
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  • WB - Latexin Antibody (Center) AP1467c
    Anti-Latexin Antibody (Center) at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - Latexin Antibody (Center) AP1467c
    Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Latexin antibody (Center) (Cat.#AP1467c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • IHC-P - Latexin Antibody (Center) AP1467c
    Formalin-fixed and paraffin-embedded human Spleen tissue reacted with Latexin antibody (Center) (Cat.#AP1467c), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - Latexin Antibody (Center) AP1467c
    Flow cytometric analysis of WiDr cells using Latexin Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, FC, WB, E
Primary Accession Q9BS40
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 25750 Da
Antigen Region 78-107 aa
Additional Information
Gene ID 56925
Other Names Latexin, Endogenous carboxypeptidase inhibitor, ECI, Protein MUM, Tissue carboxypeptidase inhibitor, TCI, LXN
Target/Specificity This Latexin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-107 amino acids from the Central region of human Latexin.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLatexin Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LXN
Function Hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. May play a role in inflammation.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in heart, prostate, ovary, kidney, pancreas, and colon, moderate or low in other tissues including brain
Research Areas
Citations (0)
citation

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Background

Latexin possesses nearly irreversible, non-competitive and potent inhibition of zinc-dependent metallocarboxypeptidases CPA1, CPA2, and CPA4. It is expressed in a neuronal subset in the cerebral cortex and cells in other neural and non-neural tissues of rat. Latexin plays a role in regional specification and/or morphogenesis of the forebrain.

References

Pallares,I., Proc. Natl. Acad. Sci. U.S.A. 102 (11), 3978-3983 (2005)

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$ 365.00
$ 140.00
Cat# AP1467c
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