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HMGB3 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HMGB3 Antibody (Center) AP14662c
    HMGB3 Antibody (Center) (Cat. #AP14662c) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the HMGB3 antibody detected the HMGB3 protein (arrow).
    detail
  • WB - HMGB3 Antibody (Center) AP14662c
    Anti-HMGB3 Antibody (Center) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • SPECIFICATION
  • CITATIONS: 1
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O15347
Other Accession O54879, P40618, Q32L31, NP_005333.2
Reactivity Human
Predicted Bovine, Chicken, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 22980 Da
Antigen Region 50-79 aa
Additional Information
Gene ID 3149
Other Names High mobility group protein B3, High mobility group protein 2a, HMG-2a, High mobility group protein 4, HMG-4, HMGB3, HMG2A, HMG4
Target/Specificity This HMGB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-79 amino acids from the Central region of human HMGB3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHMGB3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HMGB3
Synonyms HMG2A, HMG4
Function Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference for non-canonical DNA structures such as single-stranded DNA. Can bend DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (By similarity). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (By similarity).
Cellular Location Nucleus {ECO:0000250|UniProtKB:P40618, ECO:0000255|PROSITE-ProRule:PRU00267}. Chromosome Cytoplasm {ECO:0000250|UniProtKB:O54879}
Tissue Location Expressed predominantly in placenta.
Research Areas
Citations ( 0 )

Background

HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).

References

Nemeth, M.J., et al. Proc. Natl. Acad. Sci. U.S.A. 103(37):13783-13788(2006)
Ross, M.T., et al. Nature 434(7031):325-337(2005)
Vaccari, T., et al. Genomics 49(2):247-252(1998)
Wilke, K., et al. Gene 198 (1-2), 269-274 (1997) :
Davis, D.L., et al. Gene 113(2):251-256(1992)

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$ 182.50
$ 70.00
Cat# AP14662c
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