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NCL Antibody (Center P291)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - NCL Antibody (Center P291) AP14641C
    Fluorescent confocal image of U251 cell stained with NCL Antibody (Center P291)(Cat#AP14641c).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NCL immunoreactivity is localized to Nucleus significantly.
    detail
  • WB - NCL Antibody (Center P291) AP14641C
    NCL Antibody (Center P291) (Cat. #AP14641c) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the NCL antibody detected the NCL protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, WB, E
Primary Accession P19338
Other Accession Q4R4J7, NP_005372.2
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 76614 Da
Antigen Region 276-304 aa
Additional Information
Gene ID 4691
Other Names Nucleolin, Protein C23, NCL
Target/Specificity This NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human NCL.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNCL Antibody (Center P291) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCL
Function Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG- 3' repeats more tightly than the telomeric single-stranded DNA 5'- TTAGGG-3' repeats.
Cellular Location Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs
Citations (0)
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Background

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

References

Ishimaru, D., et al. J. Biol. Chem. 285(35):27182-27191(2010)
Tulchin, N., et al. Am. J. Pathol. 176(3):1203-1214(2010)
Strang, B.L., et al. J. Virol. 84(4):1771-1784(2010)
Bertrand, L., et al. J. Virol. 84(1):109-118(2010)
Jerke, U., et al. PLoS ONE 4 (12), E8302 (2009) :

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$ 365.00
$ 140.00
Cat# AP14641C
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