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GTF2E1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GTF2E1 Antibody (N-term) AP14432a
    GTF2E1 Antibody (N-term) (Cat. #AP14432a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the GTF2E1 antibody detected the GTF2E1 protein (arrow).
    detail
  • IHC-P - GTF2E1 Antibody (N-term) AP14432a
    GTF2E1 Antibody (N-term) (AP14432a)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GTF2E1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P29083
Other Accession A6QLI8, NP_005504.2
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 49452 Da
Antigen Region 93-121 aa
Additional Information
Gene ID 2960
Other Names General transcription factor IIE subunit 1, General transcription factor IIE 56 kDa subunit, Transcription initiation factor IIE subunit alpha, TFIIE-alpha, GTF2E1, TF2E1
Target/Specificity This GTF2E1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-121 amino acids from the N-terminal region of human GTF2E1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGTF2E1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GTF2E1
Synonyms TF2E1
Function Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Cellular Location Nucleus
Research Areas
Citations (0)
citation

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Background

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

References

Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)
Okuda, M., et al. EMBO J. 27(7):1161-1171(2008)
Di Lello, P., et al. Proc. Natl. Acad. Sci. U.S.A. 105(1):106-111(2008)
Jawhari, A., et al. EMBO Rep. 7(5):500-505(2006)
Okuda, M., et al. J. Biol. Chem. 279(49):51395-51403(2004)

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$ 365.00
$ 140.00
Cat# AP14432a
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