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HNMT Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, IF, E |
|---|---|
| Primary Accession | P50135 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 33295 Da |
| Antigen Region | 29-58 aa |
| Gene ID | 3176 |
|---|---|
| Other Names | Histamine N-methyltransferase, HMT, HNMT |
| Target/Specificity | This HNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human HNMT. |
| Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | HNMT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HNMT |
|---|---|
| Function | Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine. |
| Cellular Location | Cytoplasm. |
Provided below are standard protocols that you may find useful for product applications.
Background
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. HNMT is the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells.
References
Aksoy, S., et al., Biochem. Biophys. Res. Commun. 219(2):548-554 (1996).
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