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ARNT Antibody (Center V528)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - ARNT Antibody (Center  V528) AP14310c
    Fluorescent image of A549 cell stained with hARNT-V528(Cat#AP14310c).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hARNT primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).hARNT immunoreactivity is localized to Nucleus significantly.
    detail
  • WB - ARNT Antibody (Center  V528) AP14310c
    ARNT Antibody (V528) (Cat. #AP14310c) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ARNT antibody detected the ARNT protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P27540
Other Accession Q9BE97, NP_848514.1, NP_001659.1
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 86636 Da
Antigen Region 513-544 aa
Additional Information
Gene ID 405
Other Names Aryl hydrocarbon receptor nuclear translocator, ARNT protein, Class E basic helix-loop-helix protein 2, bHLHe2, Dioxin receptor, nuclear translocator, Hypoxia-inducible factor 1-beta, HIF-1-beta, HIF1-beta, ARNT, BHLHE2
Target/Specificity This ARNT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-544 amino acids from the Central region of human ARNT.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARNT Antibody (Center V528) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARNT (HGNC:700)
Synonyms BHLHE2
Function Required for activity of the AHR. Upon ligand binding, AHR translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE). Not required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding (PubMed:34521881). The complex initiates transcription of genes involved in the regulation of a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (Probable). The heterodimer binds to core DNA sequence 5'- TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409).
Cellular Location Nucleus.
Research Areas
Citations (0)
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Background

The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1. A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Alternative splicing results in multiple transcript variants.

References

Otsubo, K., et al. Cancer Genet. Cytogenet. 202(1):22-26(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Kewley, R.J., et al. Biochem. Biophys. Res. Commun. 338(1):660-667(2005)
Kewley, R.J., et al. Biochem. Biophys. Res. Commun. 338(1):660-667(2005)

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$ 182.50
$ 70.00
Cat# AP14310c
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