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CCL15 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CCL15 Antibody (C-term) AP14217b
    CCL15 Antibody (C-term) (Cat. #AP14217b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CCL15 antibody detected the CCL15 protein (arrow).
    detail
  • IHC-P - CCL15 Antibody (C-term) AP14217b
    CCL15 Antibody (C-term) (AP14217b)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCL15 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q16663
Other Accession NP_116741.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 12236 Da
Antigen Region 71-100 aa
Additional Information
Gene ID 6359
Other Names C-C motif chemokine 15, Chemokine CC-2, HCC-2, Leukotactin-1, LKN-1, MIP-1 delta, Macrophage inflammatory protein 5, MIP-5, Mrp-2b, NCC-3, Small-inducible cytokine A15, CCL15(22-92), CCL15(25-92), CCL15(29-92), CCL15, MIP5, NCC3, SCYA15
Target/Specificity This CCL15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-100 amino acids from the C-terminal region of human CCL15.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCL15 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCL15
Synonyms MIP5, NCC3, SCYA15
Function Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the CCL15.
Cellular Location Secreted.
Tissue Location Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients.
Research Areas
Citations (0)
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Background

This gene, chemokine (C-C motif) ligand 15, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene is chemotactic for T cells and monocytes and induces N-acetyl-beta-D-glucosaminidase release in monocytes. It induces changes in intracellular calcium concentration in monocytes and is thought to act through the CCR1 receptor. Read-through transcripts are expressed that include exons from the downstream cytokine gene, chemokine (C-C motif) ligand 14, and are represented as GeneID: 348249.

References

Kitamura, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(29):13063-13068(2010)
Cho, J.E., et al. Mol. Cells 29(1):35-39(2010)
Kwon, S.H., et al. Nutr Res Pract 2(2):134-137(2008)
Zhang, Z., et al. Protein Sci. 13(10):2819-2824(2004)
Escher, S.E., et al. J. Pept. Res. 63(1):36-47(2004)

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$ 182.50
$ 70.00
Cat# AP14217b
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