EPAS1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q99814 |
Other Accession | NP_001421.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 96459 Da |
Antigen Region | 577-605 aa |
Gene ID | 2034 |
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Other Names | Endothelial PAS domain-containing protein 1, EPAS-1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73, bHLHe73, HIF-1-alpha-like factor, HLF, Hypoxia-inducible factor 2-alpha, HIF-2-alpha, HIF2-alpha, Member of PAS protein 2, PAS domain-containing protein 2, EPAS1, BHLHE73, HIF2A, MOP2, PASD2 |
Target/Specificity | This EPAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 577-605 amino acids from the C-terminal region of human EPAS1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | EPAS1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | EPAS1 |
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Synonyms | BHLHE73, HIF2A, MOP2, PASD2 |
Function | Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD (By similarity). |
Cellular Location | Nucleus {ECO:0000250|UniProtKB:P97481, ECO:0000255|PROSITE-ProRule:PRU00981}. Nucleus speckle {ECO:0000250|UniProtKB:P97481}. Note=Colocalizes with HIF3A in the nucleus and speckles. {ECO:0000250|UniProtKB:P97481} |
Tissue Location | Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4.
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Yi, X., et al. Science 329(5987):75-78(2010)
Bougatef, F., et al. PLoS ONE 5 (8), E12265 (2010) :
Hossein Ghaderian, S.M., et al. Pathology 42(5):446-453(2010)
Mowat, F.M., et al. PLoS ONE 5 (6), E11103 (2010) :
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