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NOS2A Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P35228 |
| Other Accession | NP_000616.3 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 131117 Da |
| Antigen Region | 108-138 aa |
| Gene ID | 4843 |
|---|---|
| Other Names | Nitric oxide synthase, inducible, Hepatocyte NOS, HEP-NOS, Inducible NO synthase, Inducible NOS, iNOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, NOS2, NOS2A |
| Target/Specificity | This NOS2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-138 amino acids from the N-terminal region of human NOS2A. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NOS2A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NOS2 (HGNC:7873) |
|---|---|
| Synonyms | NOS2A |
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7504305, PubMed:7531687, PubMed:7544004, PubMed:7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed:19688109). |
| Cellular Location | Cytoplasm, cytosol. Note=Localizes as discrete foci scattered throughout the cytosol and in the presence of SPSB1 and SPSB4, exhibits a more diffuse cytosolic localization. |
| Tissue Location | Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets Expressed in chondrocytes (PubMed:7504305) |
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Provided below are standard protocols that you may find useful for product applications.
Background
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
References
Ryk, C., et al. J. Urol. 184(5):2150-2157(2010)
Planche, T., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 299 (5), R1248-R1253 (2010) :
Feng, C., et al. FEBS Lett. 584(20):4335-4338(2010)
Mokrzycka, M., et al. Folia Histochem. Cytobiol. 48(2):191-196(2010)
Tupitsyna, T.V., et al. Mol. Gen. Mikrobiol. Virusol. 3, 3-7 (2010) :
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