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CDKN2B Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - CDKN2B Antibody (C-term) AP14138b
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells labeling CDKN2B with AP14138b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus and Cytoplasm staining on Hela cell line. The nuclear counter stain is DAPI (blue).
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  • WB - CDKN2B Antibody (C-term) AP14138b
    All lanes : Anti-CDKN2B Antibody (C-term) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: HACAT whole cell lysate Lane 4: Hela whole cell lysate Lane 5: HepG2 whole cell lysate Lane 6: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - CDKN2B Antibody (C-term) AP14138b
    AP14138b staining CDKN2B in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/100) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • IHC-P - CDKN2B Antibody (C-term) AP14138b
    AP14138b staining CDKN2B in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • FC - CDKN2B Antibody (C-term) AP14138b
    Overlay histogram showing Hela cells stained with AP14138b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP14138b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, WB, FC, IHC-P, E
Primary Accession P42772
Other Accession NP_004927.2, NP_511042.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 14722 Da
Antigen Region 103-131 aa
Additional Information
Gene ID 1030
Other Names Cyclin-dependent kinase 4 inhibitor B, Multiple tumor suppressor 2, MTS-2, p14-INK4b, p15-INK4b, p15INK4B, CDKN2B, MTS2
Target/Specificity This CDKN2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-131 amino acids from the C-terminal region of human CDKN2B.
Dilution IF~~1:25
WB~~1:2000
IHC-P~~1:250
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDKN2B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDKN2B
Synonyms MTS2
Function Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.
Cellular Location Cytoplasm. Note=Also found in the nucleus
Tissue Location Isoform 2 is expressed in normal (keratinocytes, fibroblasts) and tumor cell lines.
Research Areas
Citations (0)
citation

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Background

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.

References

Camacho, C.V., et al. Carcinogenesis 31(10):1889-1896(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Pechlivanis, S., et al. Arterioscler. Thromb. Vasc. Biol. 30(9):1867-1872(2010)
Heni, M., et al. Diabetes (2010) In press :
Roder, C., et al. Childs Nerv Syst (2010) In press :

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$ 182.50
$ 70.00
Cat# AP14138b
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