BBC3 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| WB, IHC-P, E |
---|---|
Primary Accession | Q9BXH1 |
Other Accession | NP_055232.1, NP_001120714.1, NP_001120713.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 20532 Da |
Antigen Region | 109-137 aa |
Gene ID | 27113 |
---|---|
Other Names | Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis, BBC3, PUMA |
Target/Specificity | This BBC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-137 amino acids from the Central region of human BBC3. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | BBC3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | BBC3 |
---|---|
Synonyms | PUMA |
Function | Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis (PubMed:11463391, PubMed:23340338). Promotes partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53, releasing the bound p53/TP53 to induce apoptosis (PubMed:23340338). Regulates ER stress-induced neuronal apoptosis (By similarity). |
Cellular Location | Mitochondrion Note=Localized to the mitochondria in order to induce cytochrome c release |
Tissue Location | Ubiquitously expressed. |

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Provided below are standard protocols that you may find useful for product applications.
Background
BBC3 is an essential mediator of p53-dependent and p53-independent apoptosis.
References
Cazanave, S.C., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 299 (1), G236-G243 (2010) :
Gurzov, E.N., et al. J. Biol. Chem. 285(26):19910-19920(2010)
Hemmati, P.G., et al. J. Mol. Med. 88(6):609-622(2010)
Gomes, N.P., et al. Genes Dev. 24(10):1022-1034(2010)
Liu, F.T., et al. Biochem. Biophys. Res. Commun. 310(3):956-962(2003)

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