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USO1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | O60763 |
| Other Accession | NP_003706.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 107895 Da |
| Antigen Region | 932-960 aa |
| Gene ID | 8615 |
|---|---|
| Other Names | General vesicular transport factor p115, Protein USO1 homolog, Transcytosis-associated protein, TAP, Vesicle-docking protein, USO1, VDP |
| Target/Specificity | This USO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 932-960 amino acids from the C-terminal region of human USO1. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | USO1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | USO1 |
|---|---|
| Synonyms | VDP |
| Function | General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. |
| Cellular Location | Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein. Note=Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes |
Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner.
References
Striegl, H., et al. PLoS ONE 5 (2), E8991 (2010) :
Merk, M., et al. J. Immunol. 182(11):6896-6906(2009)
Mukherjee, S., et al. J. Biol. Chem. 284(3):1709-1717(2009)
Striegl, H., et al. PLoS ONE 4 (2), E4656 (2009) :
Guo, Y., et al. Mol. Biol. Cell 19(7):2830-2843(2008)
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