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Mouse Itk Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Mouse Itk Antibody (N-term) AP13916a
    Mouse Itk Antibody (N-term) (Cat. #AP13916a) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Itk antibody detected the Itk protein (arrow).
    detail
  • WB - Mouse Itk Antibody (N-term) AP13916a
    Mouse Itk Antibody (N-term) (Cat. #AP13916a) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Itk antibody detected the Itk protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q03526
Other Accession Q08881, NP_034713.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 72292 Da
Antigen Region 24-53 aa
Additional Information
Gene ID 16428
Other Names Tyrosine-protein kinase ITK/TSK, IL-2-inducible T-cell kinase, Kinase EMT, Kinase TLK, T-cell-specific kinase, Itk, Emt, Tlk, Tsk
Target/Specificity This Mouse Itk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of mouse Itk.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Itk Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Itk
Synonyms Emt, Tlk, Tsk
Function Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Required for TCR- mediated calcium response in gamma-delta T-cells, may also be involved in the modulation of the transcriptomic signature in the Vgamma2- positive subset of immature gamma-delta T-cells (PubMed:23562159). Phosphorylates TBX21 at 'Tyr-525' and mediates its interaction with GATA3 (PubMed:15662016).
Cellular Location Cytoplasm. Nucleus. Note=Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation.
Tissue Location Is detected in the thymus, lymph node and very faintly in the spleen, but is not detected in the liver, lung, kidney, heart, brain, intestine or testis. Expressed in T-lymphocytes and mast cells. It may also be expressed in natural killer cells
Research Areas
Citations (0)
citation

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Background

Itk may play a role in T-cell development, potentially in thymic selection.

References

Joseph, R.E., et al. J. Mol. Biol. 403(2):231-242(2010)
Dierks, C., et al. Cancer Res. 70(15):6193-6204(2010)
Grasis, J.A., et al. Mol. Cell. Biol. 30(14):3596-3609(2010)
Xia, M., et al. J. Immunol. 184(12):6807-6814(2010)
Sahu, N., et al. PLoS ONE 5 (6), E11348 (2010) :

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$ 365.00
$ 140.00
Cat# AP13916a
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