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POU2F2 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P09086 |
| Other Accession | Q29013, Q00196, NP_002689.1 |
| Reactivity | Human |
| Predicted | Mouse, Pig |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 51209 Da |
| Antigen Region | 10-39 aa |
| Gene ID | 5452 |
|---|---|
| Other Names | POU domain, class 2, transcription factor 2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, Octamer-binding protein 2, Oct-2, Octamer-binding transcription factor 2, OTF-2, POU2F2, OCT2, OTF2 |
| Target/Specificity | This POU2F2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-39 amino acids from the N-terminal region of human POU2F2. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | POU2F2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | POU2F2 (HGNC:9213) |
|---|---|
| Synonyms | OCT2, OTF2 |
| Function | Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:2904654, PubMed:7859290). Regulates IL6 expression in B cells with POU2AF1 (By similarity). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression (PubMed:2901913, PubMed:2904654). Modulates transcription transactivation by NR3C1, AR and PGR (PubMed:10480874). |
| Cellular Location | Cytoplasm. Nucleus. |
| Tissue Location | Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
POU2F2 is a transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
References
Galetti, M., et al. Biochem. Pharmacol. 80(2):179-187(2010)
Bailey, S.D., et al. Diabetes Care (2010) In press :
Advani, A.S., et al. Leuk. Lymphoma 51(4):606-612(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Filipski, K.K., et al. Clin. Pharmacol. Ther. 86(4):396-402(2009)
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