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RAB1B Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - RAB1B Antibody (C-term) AP13810b
    All lanes : Anti-RAB1B Antibody (C-term) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: T47D whole cell lysate Lane 5: U-87 MG whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: rat liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - RAB1B Antibody (C-term) AP13810b
    All lanes : Anti-RAB1B Antibody (C-term) at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: T47D whole cell lysate Lane 5: U-87MG whole cell lysate Lane 6: Mouse liver lysate Lane 7: Rat liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P-Leica - RAB1B Antibody (C-term) AP13810b
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using AP13810b performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P-Leica - RAB1B Antibody (C-term) AP13810b
    Immunohistochemical analysis of paraffin-embedded human liver tissue using AP13810b performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P-Leica, WB, E
Primary Accession Q9H0U4
Other Accession Q92928, P10536, Q06AU7, Q9D1G1, Q4R8X3, Q2HJH2, NP_112243.1
Reactivity Human, Mouse, Rat
Predicted Bovine, Monkey, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 22171 Da
Antigen Region 166-194 aa
Additional Information
Gene ID 81876
Other Names Ras-related protein Rab-1B, RAB1B
Target/Specificity This RAB1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-194 amino acids from the C-terminal region of human RAB1B.
Dilution WB~~1:1000
IHC-P-Leica~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB1B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB1B
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:20545908, PubMed:9437002). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:9437002). Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum (PubMed:20545908). Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments (By similarity). Required to modulate the compacted morphology of the Golgi (PubMed:26209634). Promotes the recruitment of lipid phosphatase MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment (By similarity).
Cellular Location Cytoplasm. Membrane; Lipid-anchor; Cytoplasmic side. Preautophagosomal structure membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:P10536}. Note=Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments (PubMed:11389151). In the GDP-form, colocalizes with GDI in the cytoplasm (PubMed:11389151). Co-localizes with MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment and to the peri- Golgi region (By similarity). {ECO:0000250|UniProtKB:P10536, ECO:0000269|PubMed:11389151}
Research Areas
Citations (0)
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Background

Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).[supplied by OMIM].

References

Yamayoshi, S., et al. J. Virol. 84(9):4816-4820(2010)
Machner, M.P., et al. Science 318(5852):974-977(2007)
Monetta, P., et al. Mol. Biol. Cell 18(7):2400-2410(2007)
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

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$ 365.00
$ 140.00
Cat# AP13810b
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