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GDI2 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P50395 |
| Other Accession | NP_001485.2, NP_001108628.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 50663 Da |
| Antigen Region | 135-164 aa |
| Gene ID | 2665 |
|---|---|
| Other Names | Rab GDP dissociation inhibitor beta, Rab GDI beta, Guanosine diphosphate dissociation inhibitor 2, GDI-2, GDI2, RABGDIB |
| Target/Specificity | This GDI2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the Central region of human GDI2. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GDI2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GDI2 |
|---|---|
| Synonyms | RABGDIB |
| Function | GDP-dissociation inhibitor preventing the GDP to GTP exchange of most Rab proteins. By keeping these small GTPases in their inactive GDP-bound form regulates intracellular membrane trafficking (PubMed:25860027). Negatively regulates protein transport to the cilium and ciliogenesis through the inhibition of RAB8A (PubMed:25860027). |
| Cellular Location | Cytoplasm. Membrane; Peripheral membrane protein |
| Tissue Location | Ubiquitous.. |
Provided below are standard protocols that you may find useful for product applications.
Background
GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously expressed. The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
References
Rikova, K., et al. Cell 131(6):1190-1203(2007)
Sun, Z.L., et al. Biochim. Biophys. Acta 1774(6):764-771(2007)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
Shin, B.K., et al. J. Biol. Chem. 278(9):7607-7616(2003)
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