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RANGAP1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P46060 |
| Other Accession | NP_002874.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 63542 Da |
| Antigen Region | 20-49 aa |
| Gene ID | 5905 |
|---|---|
| Other Names | Ran GTPase-activating protein 1, RanGAP1, RANGAP1, KIAA1835, SD |
| Target/Specificity | This RANGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-49 amino acids from the N-terminal region of human RANGAP1. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RANGAP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RANGAP1 |
|---|---|
| Synonyms | KIAA1835, SD |
| Function | GTPase activator for RAN (PubMed:16428860, PubMed:8146159, PubMed:8896452). Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export (PubMed:27160050, PubMed:8896452). Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export (PubMed:27160050). |
| Cellular Location | Cytoplasm. Nucleus, nucleoplasm. Nucleus envelope. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic during interphase Detected at the nuclear envelope during interphase (PubMed:11854305, PubMed:15037602). Targeted to the nuclear pores after sumoylation (PubMed:11854305). During mitosis, associates with mitotic spindles, but is essentially not detected at the spindle poles (PubMed:11854305, PubMed:15037602). Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase (PubMed:11854305). Mitotic location also requires sumoylation (PubMed:11854305). |
| Tissue Location | Highly expressed in brain, thymus and testis. |
Provided below are standard protocols that you may find useful for product applications.
Background
RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.
References
Zhang, J., et al. Biochem. Biophys. Res. Commun. 375(2):252-255(2008)
Zuccolo, M., et al. EMBO J. 26(7):1853-1864(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Vertegaal, A.C., et al. Mol. Cell Proteomics 5(12):2298-2310(2006)
Vertegaal, A.C., et al. Mol. Cell Proteomics 5(12):2298-2310(2006)
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