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NAPSA Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NAPSA Antibody (C-term) AP13772b
    NAPSA Antibody (C-term) (Cat. #AP13772b) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the NAPSA antibody detected the NAPSA protein (arrow).
    detail
  • IHC-P - NAPSA Antibody (C-term) AP13772b
    NAPSA Antibody (C-term) (Cat. #AP13772b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NAPSA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession O96009
Other Accession NP_004842.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 45387 Da
Antigen Region 368-397 aa
Additional Information
Gene ID 9476
Other Names Napsin-A, 3423-, Aspartyl protease 4, ASP4, Asp 4, Napsin-1, TA01/TA02, NAPSA, NAP1, NAPA
Target/Specificity This NAPSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 368-397 amino acids from the C-terminal region of human NAPSA.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNAPSA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NAPSA
Synonyms NAP1, NAPA
Function May be involved in processing of pneumocyte surfactant precursors.
Cellular Location Secreted.
Tissue Location Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes
Research Areas
Citations (0)
citation

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Background

The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. [provided by RefSeq].

References

Bishop, J.A., et al. Hum. Pathol. 41(1):20-25(2010)
Woischnik, M., et al. Eur. Respir. J. 31(6):1197-1204(2008)
Ueno, T., et al. Lab. Invest. 88(3):256-263(2008)
Innocenti, M., et al. Nat. Cell Biol. 6(4):319-327(2004)
Brasch, F., et al. J. Biol. Chem. 278(49):49006-49014(2003)

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$ 182.50
$ 70.00
Cat# AP13772b
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