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ATG9B Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ATG9B Antibody (C-term) AP13649b
    ATG9B Antibody (C-term) (Cat. #AP13649b) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the ATG9B antibody detected the ATG9B protein (arrow).
    detail
  • IHC-P - ATG9B Antibody (C-term) AP13649b
    ATG9B Antibody (C-term) (Cat. #AP13649b)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ATG9B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q674R7
Other Accession NP_775952.4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 101019 Da
Antigen Region 892-921 aa
Additional Information
Gene ID 285973
Other Names Autophagy-related protein 9B, APG9-like 2, Nitric oxide synthase 3-overlapping antisense gene protein, Protein sONE, ATG9B, APG9L2, NOS3AS
Target/Specificity This ATG9B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 892-921 amino acids from the C-terminal region of human ATG9B.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATG9B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG9B
Function Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 (ATG2A or ATG2B) from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion (By similarity). In addition to autophagy, also plays a role in necrotic cell death (By similarity).
Cellular Location Preautophagosomal structure membrane; Multi-pass membrane protein. Note=Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool (PubMed:18936157). The starvation-induced redistribution depends on ULK1 and ATG13 (PubMed:18936157).
Tissue Location Highly expressed in placenta (trophoblast cells) and pituitary gland. Not expressed in vascular endothelial
Research Areas
Citations (0)
citation

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Background

ATG9B plays a role in autophagy (By similarity).

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Han, S., et al. Hum. Immunol. 71(7):727-730(2010) Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010) Webber, J.L., et al. FEBS Lett. 584(7):1319-1326(2010) Ban, H.J., et al. BMC Genet. 11, 26 (2010) :

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$ 365.00
$ 140.00
Cat# AP13649b
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