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SYP Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SYP Antibody (C-term) AP13606b
    Western blot analysis of SYP (arrow) using rabbit polyclonal SYP Antibody (C-term) (Cat. #AP13606b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SYP gene.
    detail
  • WB - SYP Antibody (C-term) AP13606b
    SYP Antibody (C-term) (Cat. #AP13606b) western blot analysis in mouse brain,cerebellum tissue lysates (35ug/lane).This demonstrates the SYP antibody detected the SYP protein (arrow).
    detail
  • WB - SYP Antibody (C-term) AP13606b
    All lanes : Anti-SYP Antibody (C-term) at 1:8000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - SYP Antibody (C-term) AP13606b
    SYP Antibody (C-term) (Cat. #AP13606b)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SYP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • IHC-P - SYP Antibody (C-term) AP13606b
    SYP Antibody (C-term) (Cat. #AP13606b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SYP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P08247
Other Accession P07825, Q62277, NP_003170.1
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 33845 Da
Antigen Region 225-253 aa
Additional Information
Gene ID 6855
Other Names Synaptophysin, Major synaptic vesicle protein p38, SYP
Target/Specificity This SYP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-253 amino acids from the C-terminal region of human SYP.
Dilution WB~~1:8000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSYP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SYP
Function Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).
Cellular Location Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Synapse, synaptosome
Tissue Location Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3 (PubMed:8838578). Expressed in the putamen (at protein level) (PubMed:17296554)
Research Areas
Citations (0)
citation

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Background

Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM].

References

Ishida, M., et al. Oncol. Rep. 22(4):733-737(2009)
Sterlacci, W., et al. Virchows Arch. 455(2):125-132(2009)
Head, E., et al. Neurobiol. Aging 30(7):1125-1134(2009)
Gulubova, M., et al. J. Gastroenterol. Hepatol. 23(10):1574-1585(2008)
Glantz, L.A., et al. Neuroscience 149(3):582-591(2007)

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$ 182.50
$ 70.00
Cat# AP13606b
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