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CD5 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P06127 |
| Other Accession | NP_055022.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 54578 Da |
| Antigen Region | 203-232 aa |
| Gene ID | 921 |
|---|---|
| Other Names | T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, CD5, CD5, LEU1 |
| Target/Specificity | This CD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-232 amino acids from the Central region of human CD5. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CD5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CD5 |
|---|---|
| Synonyms | LEU1 |
| Function | Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL (PubMed:1384049, PubMed:1385158). Mechanistically, co- engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation (PubMed:23376399). Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production (PubMed:27499044). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P13379}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P13379} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
CD5 may act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.
References
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Yoshino, N., et al. Ann Thorac Cardiovasc Surg 15(5):324-327(2009)
Garaud, S., et al. Ann. N. Y. Acad. Sci. 1173, 280-285 (2009) :
Liang, X.S., et al. Br. J. Haematol. 146(4):418-423(2009)
Vera, J., et al. Proc. Natl. Acad. Sci. U.S.A. 106(5):1506-1511(2009)
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