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RAB5C Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RAB5C Antibody (C-term) AP13450b
    All lanes : Anti-RAB5C Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - RAB5C Antibody (C-term) AP13450b
    RAB5C Antibody (C-term) (Cat. #AP13450b)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RAB5C Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P51148
Other Accession Q58DS9, NP_004574.2, NP_958842.1
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 23483 Da
Antigen Region 178-207 aa
Additional Information
Gene ID 5878
Other Names Ras-related protein Rab-5C, L1880, RAB5L, RAB5C, RABL
Target/Specificity This RAB5C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the C-terminal region of human RAB5C.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB5C Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB5C
Synonyms RABL
Function Protein transport. Probably involved in vesicular traffic.
Cellular Location Cell membrane {ECO:0000250|UniProtKB:P20339}; Lipid-anchor {ECO:0000250|UniProtKB:P20339}; Cytoplasmic side {ECO:0000250|UniProtKB:P20339}. Early endosome membrane {ECO:0000250|UniProtKB:P20339}; Lipid-anchor {ECO:0000250|UniProtKB:P20339}. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Research Areas
Citations (0)
citation

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Background

Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]).

References

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)
Merithew, E., et al. J. Biol. Chem. 278(10):8494-8500(2003)
Clemens, D.L., et al. Infect. Immun. 68(5):2671-2684(2000)
Bucci, C., et al. Biochem. Biophys. Res. Commun. 258(3):657-662(1999)

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$ 182.50
$ 70.00
Cat# AP13450b
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