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SGPP1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P-Leica, WB, E |
|---|---|
| Primary Accession | Q9BX95 |
| Other Accession | NP_110418.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 49108 Da |
| Antigen Region | 13-42 aa |
| Gene ID | 81537 |
|---|---|
| Other Names | Sphingosine-1-phosphate phosphatase 1, SPPase1, Spp1, hSPP1, hSPPase1, 313-, Sphingosine-1-phosphatase 1, SGPP1 |
| Target/Specificity | This SGPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human SGPP1. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 IHC-P-Leica~~1:500 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SGPP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SGPP1 (HGNC:17720) |
|---|---|
| Function | Specifically dephosphorylates sphingosine 1-phosphate (S1P), dihydro-S1P, and phyto-S1P. Does not act on ceramide 1-phosphate, lysophosphatidic acid or phosphatidic acid (PubMed:16782891). Sphingosine-1-phosphate phosphatase activity is needed for efficient recycling of sphingosine into the sphingolipid synthesis pathway (PubMed:11756451, PubMed:12815058, PubMed:16782891). Regulates the intracellular levels of the bioactive sphingolipid metabolite S1P that regulates diverse biological processes acting both as an extracellular receptor ligand or as an intracellular second messenger (PubMed:11756451, PubMed:12815058, PubMed:16782891). Involved in efficient ceramide synthesis from exogenous sphingoid bases. Converts S1P to sphingosine, which is readily metabolized to ceramide via ceramide synthase. In concert with sphingosine kinase 2 (SphK2), recycles sphingosine into ceramide through a phosphorylation/dephosphorylation cycle (By similarity). Regulates endoplasmic-to-Golgi trafficking of ceramides, resulting in the regulation of ceramide levels in the endoplasmic reticulum, preferentially long-chain ceramide species, and influences the anterograde membrane transport of both ceramide and proteins from the endoplasmic reticulum to the Golgi apparatus (PubMed:16782891). The modulation of intracellular ceramide levels in turn regulates apoptosis (By similarity). Via S1P levels, modulates resting tone, intracellular Ca(2+) and myogenic vasoconstriction in resistance arteries (PubMed:18583713). Also involved in unfolded protein response (UPR) and ER stress-induced autophagy via regulation of intracellular S1P levels (PubMed:18583713, PubMed:20798685). Involved in the regulation of epidermal homeostasis and keratinocyte differentiation (By similarity). |
| Cellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane {ECO:0000250|UniProtKB:Q9JI99}; Multi-pass membrane protein |
| Tissue Location | Ubiquitous, with the strongest level in placenta and kidney. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides (Mandala et al., 2000 [PubMed 10859351]; Le Stunff et al., 2007 [PubMed 17895250]).[supplied by OMIM].
References
Hicks, A.A., et al. PLoS Genet. 5 (10), E1000672 (2009) :
Le Stunff, H., et al. J. Biol. Chem. 282(47):34372-34380(2007)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Giussani, P., et al. Mol. Cell. Biol. 26(13):5055-5069(2006)
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