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NAE1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q13564 |
| Other Accession | Q9Z1A5, Q4R3L6, NP_001018170.1, NP_003896.1 |
| Reactivity | Human |
| Predicted | Monkey, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 60246 Da |
| Antigen Region | 172-200 aa |
| Gene ID | 8883 |
|---|---|
| Other Names | NEDD8-activating enzyme E1 regulatory subunit, Amyloid beta precursor protein-binding protein 1, 59 kDa, APP-BP1, Amyloid protein-binding protein 1, Proto-oncogene protein 1, NAE1, APPBP1 |
| Target/Specificity | This NAE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 172-200 amino acids from the Central region of human NAE1. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NAE1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NAE1 |
|---|---|
| Synonyms | APPBP1 |
| Function | Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. The covalent attachment of NEDD8 to target proteins is known as 'neddylation' and the process is involved in the regulation of cell growth, viability and development. |
| Cellular Location | Cell membrane. Note=Colocalizes with APP in lipid rafts |
| Tissue Location | Ubiquitous in fetal tissues. Expressed throughout the adult brain. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene.
References
Jung, A.C., et al. Int. J. Cancer 126(8):1882-1894(2010)
Park, Y., et al. Biochem. Biophys. Res. Commun. 374(2):294-298(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Li, T., et al. Arch. Biochem. Biophys. 453(1):70-74(2006)
Norman, J.A., et al. Biochemistry 45(9):3014-3019(2006)
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