PLEKHM1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | Q9Y4G2 |
Other Accession | NP_055613.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 117443 Da |
Antigen Region | 50-78 aa |
Gene ID | 9842 |
---|---|
Other Names | Pleckstrin homology domain-containing family M member 1, PH domain-containing family M member 1, 162 kDa adapter protein, AP162, PLEKHM1, KIAA0356 |
Target/Specificity | This PLEKHM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids from the N-terminal region of human PLEKHM1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PLEKHM1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PLEKHM1 (HGNC:29017) |
---|---|
Synonyms | KIAA0356 |
Function | Acts as a multivalent adapter protein that regulates Rab7- dependent and HOPS complex-dependent fusion events in the endolysosomal system and couples autophagic and the endocytic trafficking pathways. Acts as a dual effector of RAB7A and ARL8B that simultaneously binds these GTPases, bringing about clustering and fusion of late endosomes and lysosomes (PubMed:25498145, PubMed:28325809). Required for late stages of endolysosomal maturation, facilitating both endocytosis- mediated degradation of growth factor receptors and autophagosome clearance. Interaction with Arl8b is a crucial factor in the terminal maturation of autophagosomes and to mediate autophagosome-lysosome fusion (PubMed:25498145). Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (By similarity). May be involved in negative regulation of endocytic transport from early endosome to late endosome/lysosome implicating its association with Rab7 (PubMed:20943950). May have a role in sialyl-lex- mediated transduction of apoptotic signals (PubMed:12820725). Involved in bone resorption (By similarity). |
Cellular Location | Autolysosome membrane. Endosome membrane. Late endosome membrane. Lysosome membrane. Note=In case of infection colocalizes with Salmonella typhimurium sifA in proximity of Salmonella-containing vacuole (SCV) (PubMed:25500191). |
Tissue Location | Expressed in placenta, liver, prostate, thymus, spleen, ovary, colon, colon carcinoma and peripheral blood lymphocytes (PBL). Weakly expressed in brain, lung, kidney, and testis. No expression in heart, skeletal muscle, pancreas and small intestine Predominantly expressed in the breast carcinoma cell line MCF-7 |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is essential for bone resorption, and may play a critical role in vesicular transport in the osteoclast. Mutations in this gene are associated with autosomal recessive osteopetrosis type 6 (OPTB6). Alternatively spliced transcript variants have been found for this gene.
References
Edwards, T.L., et al. Ann. Hum. Genet. 74(2):97-109(2010)
Del Fattore, A., et al. J. Bone Miner. Res. 23(3):380-391(2008)
Van Wesenbeeck, L., et al. J. Clin. Invest. 117(4):919-930(2007)
Hartel-Schenk, S., et al. Glycoconj. J. 18 (11-12), 915-923 (2001) :
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.