NIT1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q86X76 |
Other Accession | Q32LH4, NP_001172023.1, NP_005591.1, NP_001172022.1, NP_001172021.1 |
Reactivity | Human |
Predicted | Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 35896 Da |
Antigen Region | 298-327 aa |
Gene ID | 4817 |
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Other Names | Nitrilase homolog 1, 35--, NIT1 |
Target/Specificity | This NIT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-327 amino acids from the C-terminal region of human NIT1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | NIT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | NIT1 |
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Function | Catalyzes the hydrolysis of the amide bond in N-(4- oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. |
Cellular Location | [Isoform 2]: Mitochondrion {ECO:0000250|UniProtKB:Q8VDK1} |
Tissue Location | Detected in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene.
References
Semba, S., et al. J. Biol. Chem. 281(38):28244-28253(2006)
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
Pekarsky, Y., et al. Proc. Natl. Acad. Sci. U.S.A. 95(15):8744-8749(1998)
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